Staff Resources & Support

NHS Greater Glasgow and Clyde Pathology Department is undergoing a digital transformation whereby glass slides are digitised and are able to be viewed on digital workstations, rather than microscopes.  This change is being undertaking in partnership with Philips Healthcare. 

The transformation will take several years to complete for histology (and longer for cytology where the technology is less mature).  We are following national guidelines and best practice recommendations from the Royal College of Pathologists (RCPath) and pathologists reporting digitally are following the RCPath validation process.  Digital Pathology is being incorporated into our quality management system and we are working towards UKAS extension to scope. 

We fully believe that this development will deliver both quality and safety benefits to our service users. 

Intra-Operative Frozen Section Requests

Pre-Booking a Frozen Section

 The requesting clinician must ensure that:

• Pathology Duty Consultant is notified of frozen section request 24 hours in advance of sample collection, via Pathology Specimen Reception on 0141 354 9513 or 0141 354 9514 (89513 or 89514)
• As per national guidance, the department will routinely process requests for frozen section analysis unless the clinician or request form indicate the patient potentially being COVID positive.
• On the day of surgery, theatre staff notify Pathology reception that the frozen section is on its way to the Laboratory.

Failure to pre-book an intra-operative frozen section may result in delays in reporting and in exceptional circumstances the request being declined.

When pre-booking an intra-operative frozen please provide the following details:

• Patient’s name and CHI number
• Requesting clinician
• Theatre name/number including hospital
• Likely time of operation
• Contact number
• Specimen type

The Consultant Pathologist will phone the result to the requesting clinician on the contact number given.

Queen Elizabeth University Hospital (QEUH)

An intra-operative frozen section service is available Monday to Friday from 9:00am to 5:00pm

Golden Jubilee National Hospital (GJNH)

An intra-operative frozen section service is provided, on site at GJNH, Monday to Thursday from 9:00am to 1:00pm. Any cases requiring a frozen section should be recorded on the GJNH theatre lists.

To arrange an intra-operative frozen section out with these times please contact the Duty Pathologist via Pathology Specimen Reception at QEUH on the telephone number below.

These specimens will be transferred to Pathology Department at QEUH for processing and should be sent by taxi to:

Specimen Reception (L3/B/021),

NHSGGC Pathology Department

Level 3, Laboratory Medicine and Facilities Management Building

Queen Elizabeth University Hospital

Tel:  0141 354 9513 (89513) or 0141 354 9514 (89514)

It is the responsibility of staff at GJNH to arrange rapid transfer of these specimens to QEUH as per local protocol.

Glasgow Royal Infirmary (GRI)

An intra-operative frozen section service is available Monday to Friday within normal laboratory working hours, from 9am to 5pm.

This Laboratory is only manned for pre-booked requests. It is, therefore, imperative that frozen section requests for onsite analysis at GRI are received by the Pathology Department more than 24 hours in advance, by phone call.

From time to time an urgent frozen section is required due to an unexpected finding at surgery. The department provides this service, but for intra-operative frozen section requested on site at GRI, a short delay is inevitable to allow staff to travel.

Alternatively the specimen can be sent to QEUH urgently by courier by telephoning:

01412113734 or 01412113674 (Transport Hub) and following the information below for Transporting Urgent Fresh Specimens.

Labelling Frozen Section Specimens

The normal requirements for labelling specimens and request forms apply to frozen sections in addition to the following criteria on the request form:

• Hospital
• Theatre
• Date and time of request.
• Requesting clinician
• Alerts to known or suspected danger of infection
• Clinical History
• Theatre contact number

Transporting Urgent Fresh Specimens

QEUH site:

Fresh (unfixed) specimens should be sent directly to:

Specimen Reception (L3/B/021),

NHSGGC Pathology Department

Level 3, Laboratory Medicine and Facilities Management Building

Queen Elizabeth University Hospital

Tel:  0141 354 9513 (89513) or 0141 354 9514 (89514)

Other Sites

Fresh (unfixed) specimens taken at other sites out with stated service provision at GJNH and GRI should be sent directly to the Pathology laboratory at QEUH by courier.

The courier should be telephoned by theatre staff, telephone Eagle Couriers on 08451231230 at least 30 minutes before the specimen is available on the day of the operation.

 The information required by Eagle couriers includes:

• Hospital site
• Theatre collection point
• Advise that this is an “Emergency Pathology Specimen”.
• Codes used for each location should be as per local protocols.
• Please telephone Pathology Specimen Reception on 01413549513 or 01413549514 (89513 or 89514) when the courier is on the way

Fresh/Urgent Paediatric Specimens

Paediatric Rectal biopsies for diagnosis of Hirschsprung’s disease via Acetylcholinesterase histochemistry must be sent fresh (not fixed) as is done for frozen sections (see above).

Please ensure that for all specimens indicative of paediatric neoplasia/cancer/tumour that the paediatric team are notified (89531) in advance of submission and the specimens are submitted fresh/unfixed as is done for frozen sections (see above).

Fresh Lymph Nodes for Suspected Lymphoma

Excised lymph nodes, for suspected lymphoma should be bisected alone their long axis at the time of removal.

Half of the specimen should be placed into a sealed container with buffered formalin. The second half of the specimen should be placed in dry container, without formalin and sent “fresh” to the Pathology Department to allow ancillary testing to be undertaken.

It is particularly important that any Danger of Infection is recorded on the request form.

Follow the instructions above for Transporting Urgent Fresh Specimens.

Orthopaedic Amputations

The orthopaedic theatre should contact Pathology Specimen Reception at least 24hrs before procedure is scheduled on 01413549513 or 01413549514 (89513 or 89514) to request that a dedicated large amputation specimen container is sent to relevant theatre.

Specimens such as amputations that have a high ratio of surface area covered by skin should be sent unfixed. If there is a surgical stocking, leave in place.

Reasons for this are:

• Poor penetration of skin by formalin
• Moving and handling considerations

To contain potential leakage, put the amputation into a large plastic bag or wrap in drapes as appropriate and then put into the amputation container.  Never use yellow or orange bags or containers as this corresponds to the code for waste incineration.

Please follow the instructions for transporting urgent fresh specimens above. It is important to advise the courier that this is a large specimen (requires van rather than motorcycle courier).

Paediatric rectal biopsies for diagnosis of Hirschsprung’s disease do not need to be sent fresh. These specimens may be sent in formalin. There is no requirement to discuss these cases with the duty pathologist.

Paediatric tonsils do not need to be sent fresh unless there is a high clinical suspicion of malignancy. These specimens may be sent in formalin. There is no requirement to discuss these cases with the duty pathologist unless there is a high clinical suspicion of malignancy.

Please ensure that for all specimens indicative of paediatric neoplasia/cancer/tumour that the day duty pathologist is notified in advance of submission and the specimens are submitted unfixed as specified by the day duty pathologist’s instructions. All fresh specimens including frozen sections must be discussed directly with the day duty pathologist in advance of submission.

Urgent Paediatric Specimens

The following procedures must be followed for all urgent paediatric fresh specimens including frozen sections, fresh tumour biopsies and fresh tumour resections.

Any queries regarding urgent specimens should be directed to the duty Paediatric pathologist by telephoning the paediatric office on 89478 and asking to speak to the duty paediatric pathologist. If advice/assistance is urgently required out of hours, please contact switchboard who can contact a paediatric pathologist. The paediatric laboratory team may be contacted on 89531.

Discussion with the duty pathologist – All Cases

All cases must be booked in advance (ideally the day before) by telephoning 89478 and discussing the case and your requirements with the duty paediatric pathologist. Please provide patient details, including name and CHI number, date of surgery, approximate time of surgery and a contact number for theatre.

The surgeon or interventionalist performing the biopsy / resection must speak directly to the duty Paediatric pathologist prior to the procedure in every case to establish the specimen requirements.  Failure to follow this procedure may render the biopsy un-interpretable.

The instructions below are general instructions and must not replace a case by case discussion with the duty pathologist.

• Tissue for frozen section should be kept dry and placed in a suitable container labelled with the patient’s details (ideally a small plastic dish wrapped in a yellow plastic bag). Do not place small biopsies on paper or wrap them in gauze or paper.
• Unless indicated otherwise by the day duty pathologist, all fresh tumour biopsies should be placed in pink tissue culture fluid for transport to the laboratory.
• Unless indicated otherwise by the day duty pathologist, all fresh tumour resections should be kept dry and placed in a suitable container labelled with the patient’s details.

Transport to the Laboratory – All Cases

All fresh paediatric specimens must be conveyed immediately in person or by porter to pathology (3^rd floor, Laboratory Medicine Building). It is the responsibility of the surgical team to arrange urgent transport of the specimen to pathology. The sample must not be sent via the POD system and must not be placed in a purple bag. It must be taken directly to pathology specimen reception on the 3^rd floor. The specimen should be marked as “Urgent. Frozen section.” Staff transporting the specimen must inform specimen reception staff that it is an urgent fresh specimen for frozen section.

When the specimen leaves theatre, theatre staff must inform the laboratory by telephoning 89531.

Paediatric Renal Biopsies

The following procedures must be followed for all paediatric renal biopsies:

Discussions with the Duty Pathologist and the Laboratory

All Renal biopsies must be discussed with the Duty Paediatric Pathologist on 89478 as well as with laboratory staff on 89531 (see below).

These specimens will be collected in theatre by a biomedical scientist who will separate the sample for immunofluorescence and electron microscopy.  Notification of such biopsies to the laboratory must be done as far in advance of the biopsy as possible by telephoning 89531. Failure to do so may result in delay since staff will have to be available to attend theatre to undertake this procedure.

The placental request form is linked here

Criteria for Examination

The local criteria for placental examination are:

Stillbirth

Miscarriage > 14/40

Birth weight < 3rd centile

Drop in fetal growth of > 2 quartiles or > 50 percentiles

Absent or reversed EDF on umbilical artery Dopplers

Spontaneous preterm delivery or prolonged preterm rupture of membranes less than 32/40

Iatrogenic preterm delivery less than 32 weeks

Severe early onset (less than 32/40) pre-eclampsia requiring iatrogenic delivery

Massive abruption with retroplacental clot

Fetal hydrops

Severe fetal distress defined as: pH<7.05 or base Excess > -12 or scalp lactate >4.8mmol/l

Severe maternal sepsis requiring adult ICU admission

Severe fetal sepsis requiring ventilation / level 3 NICU admission

Caesarean hysterectomy for morbidly adherent placenta and

Monochorionic twins with twin to twin transfusion.

Required Data Terms

Listed below are the data items that are required for pathological examination of placentas. Obligatory data items are in bold and the remainder should be added as required. Please include these data items on the pathology request form accompanying the placentas.

Please write clearly on the forms

· Mother’s name

· Mother’s date of birth

· Address

· Hospital Number and CHI number

· Date of delivery

· Date placenta sent for pathology examination

· Referring Hospital

· Referring consultant

· Midwife or trainee doctor’s name and contact extension / bleep number

· Gravida and Parity

· Gestation

· Indication for examination (see below)

· Livebirth (Y/N):

· Birth Weight and Centile:

· Mode of delivery:

In cases of stillbirth / late miscarriage, page 2 of the placenta form must be completed by a doctor of ST7 or consultant level. Please provide the following information:

Presence or absence of maceration

Interval between IUD diagnosis and delivery

Additional clinical details including any relevant antenatal history

Placentas not Requiring Pathological Examination

Any placentas not indicated for placental examination by our local protocol do not need to be sent for pathology. If sent to pathology, they will be stored with no initial examination. A brief report indicating the placenta has been stored will be issued shortly after the placenta is received (generally within a fortnight). These placentas will be retained for 3 months in the pathology department prior to disposal. Full macroscopic examination with histology will only be performed on request with additional clinical details indicating why such examination is required.

Placentas with the following are not indicated for examination and do not need to be sent to pathology unless there are additional relevant indications:

• Pre-eclampsia with onset after 32/40 and not requiring iatrogenic delivery
• Maternal sepsis not requiring adult ICU admission
• Maternal pyrexia
• Possible abruption
• Fetal distress not fulfilling the following criteria: pH<7.05 or base Excess > -12 or scalp lactate >4.8mmol/l
• Maternal Group B streptococcus
• Maternal diabetes or other maternal disease with normal pregnancy outcome
• Known trisomy 13, 18, 21 / Turners
• Congenital anomaly
• Uncomplicated twin pregnancy
• Twin placentas for assessment of chorionicity
• “Gritty” placenta
• Placenta praevia
• Post-partum haemorrhage
• Polyhydramnios
• History of previous molar pregnancy
• Cholestasis
• Hepatitis B/C, HIV
• Single umbilical artery
• Uncomplicated velamentous cord
• Placenta with accessory lobe
• Normal pregnancy

Test Name	Specimen/Container Requirements	Further Information:
Electron Microscopy	Specimens for electron microscopic analysis should ideally be no larger than 1-2 mm3 and placed into a vial of fresh 2% Glutaraldehyde fixative as soon as possible after specimen retrieval. Blood/Fluid Specimens should be discussed with the EM unit directly. (0141 3549420 or 01413549422). If specimens are sent through the post they must comply with post office regulations. EM sampling from a histology wax block must be accompanied by a representative light microscopy slide.	Freshly prepared glutaraldehyde has a 7 day shelf life and must be stored to 4-80c prior to use. This fixative is available with advance notice from: Electron Microscopy Unit, Pathology Department, Level 2, Laboratory Medicine and Facilities Management Building, Queen Elizabeth University Hospital, 1345 Govan Rd, Glasgow, G51 4TF (0141 3549420 or 01413549422). All unused Glutaraldehyde should be returned to the EM unit for disposal. Samples to be placed in a purple specimen bag.
Primary Cilial Diskenesia Service	Specimens are only accepted in the laboratory if taken from the clinic Specimens should be placed into M199 media with penicillin-streptomycin added – supplied by the EM laboratory.	Contacts for more info about the service and clinic.:  Paul French Jennifer Sweeney Anne Devenny

Specimens for EM analysis should ideally be no larger than 1-2mm³ and placed into a vial of fresh 2% Glutaraldehyde fixative as soon as possible after specimen retrieval apart from blood/fluid specimens which must be discussed with EM unit directly.

Freshly prepared glutaraldehyde has a seven-day shelf life and must be stored between 4-8⁰c prior to use. This fixative is available with advanced notice from the Electron Microscopy Unit, NHSGGC Pathology Department, Level 2, Facilities Management and Laboratory Medicine Building, Queen Elizabeth University Hospital, 1345 Govan Road, Glasgow, G51 4TF (0141 3549422).

Each specimen must be accompanied by a request form with the minimum of 3 matching patient identifiers.

Due to the small size of the specimen container, there is limited space for patient’s details but at least the patients CHI and first and surname must be provided on the specimen container.

Requests for urgent results should be clearly marked on the request form and contact telephone or bleep numbers provided. The consultant pathologist should be notified first by telephone, if an urgent result is required.

Specimens should be sent for EM analysis in the same manner as routine histopathology specimens.

If specimens are sent through the post they must comply with post office regulations. EM sampling from a histological wax block must be accompanied by a representative light microscopy slide.

All unused Glutaraldehyde should be returned to the EM unit for disposal.

EM Analysis should normally be available within 5-12 days once the sample has been received by the department for processing. This may change due to service provision. If appropriate any samples marked as urgent will have an accelerated turnaround.

Sample results are affected by a number of factors including delay in fixation, use of inappropriate fixative/old fixative and incorrect sample size (1-2mm³ is recommended; if a sample is too big this can cause sub-optimal fixation, if too small will not give a good representation of morphology).

Name Of Test	Specimen/Container Requirements:	Further Information:
Respiratory: Sputum Bronchial Aspirates, Washings, Lavages, Brushings	Do not fix sample in formalin, please use saline. Please use a 30 ml white topped universal container or trap without the tubing See below for specimens requiring a differential cell count. Please send the specimen immediately. If this is not possible, the specimen(s) can be refrigerated overnight but must be delivered at the earliest opportunity the next day.	Samples to be placed in a purple specimen bag.   Brushings should be sent in preservcyt vial available from the laboratory: Cytology Department, Level 3, Laboratory Medicine and Facilities Management Building, Queen Elizabeth University Hospital, 1345 Govan Rd, Glasgow, G51 4TF (0141 354 9569).
Bronchial Differential Cell Counts	Specimens requiring a differential cell count must be clearly described/marked on the request form. Please use a 30 ml white topped universal container or trap without the tubing. Specimens for bronchial differential cell counts must be lavages in saline (the test requires mucoid material). Please send the specimen immediately. If this is not possible, the specimen(s) can be refrigerated overnight but must be delivered at the earliest opportunity the next day.	Differential cell counts cannot be performed on patients who are positive for COVID-19   Samples to be placed in a purple specimen bag.
Pancreatic Brushings	Do not fix sample in formalin, please use Preservcyt solution. Please send the specimen immediately. If this is not possible, the specimen(s) can be refrigerated overnight but must be delivered at the earliest opportunity the next day.	Preservcyt solution is available with advance notice from: Cytology Department, Level 3, Laboratory Medicine and Facilities Management Building, Queen Elizabeth University Hospital, 1345 Govan Rd, Glasgow, G51 4TF (0141 354 9569). Samples to be placed in a purple specimen bag.
Fine Needle Aspirate (FNA): Head & Neck, Breast, Lymph Node etc.	FNA specimens should be sent on 4 glass slides: 2 slides air dried and 2 slides treated with Cytofixx solution. Please send us 2 alcohol fixed and 2 air dried slides. More information can be found in the document: Fine Needle Aspirate Labelling Guide for Cytology Users FNA specimens do not need to be refrigerated Please contact Dr Van Der Horst (01413549553) if FNA demonstration s required (Head & Neck) For FNA of Breast, please send 2 air dried slides	Cytofixx solution is available with advance notice from: Cytology Department, Level 3, Laboratory Medicine and Facilities Management Building, Queen Elizabeth University Hospital, 1345 Govan Rd, Glasgow, G51 4TF (0141 354 9569). Samples to be placed in a purple specimen bag.
Endobronchial Ultrasound Guided Transbronchial Needle Aspirate (EBUS-TBNA) Endoscopic Ultrasound Guided Fine Needle Aspirate (EUS FNA): Pancreas, Liver	Do not fix sample in formalin, please use Preservcyt vials.	Preservcyt vials are available with advance notice from: Cytology Department, Level 3, Laboratory Medicine and Facilities Management Building, Queen Elizabeth University Hospital, 1345 Govan Rd, Glasgow, G51 4TF (0141 354 9569). Samples to be placed in a purple specimen bag.
Joint (Synovial) Fluid Analysis Gout Crystal Analysis	Do not fix sample in formalin, please send fresh and with no additives. Please use a 30 ml white topped universal container Please do not add EDTA	This test has been fully validated within the cytology department but is not UKAS accredited. Samples to be placed in a purple specimen bag.
Serous Effusions	Do not fix sample in formalin, please send fresh and with no additives. The volume of sample sent should be a minimum of 75ml if possible, but preferably no more than 150ml. Unless the sample is a peritoneal washing, for these a larger volume is required, we recommend at least 300 ml. Please send the specimen immediately. If this is not possible, the specimen(s) can be refrigerated overnight but must be delivered at the earliest opportunity the next day.	Samples to be placed in a purple specimen bag.
Urine Samples	Do not fix sample in formalin, please send fresh and with no additives. Do not send a urine sample taken early in the morning or shortly after waking (midstream, not first in the morning). The volume of sample sent should preferably be 50ml Please send the specimen immediately. If this is not possible, the specimen(s) can be refrigerated overnight but must be delivered at the earliest opportunity the next day.	Samples to be placed in a purple specimen bag.
Cerebrospinal Fluid (CSF)	Do not fix sample in formalin, please send fresh and with no additives. A volume of at least 2ml is required. Please send the specimen immediately.	Samples to be placed in a purple specimen bag.
Flow Cytometry	Do not send to Cytology Department. Send to Haemato-Oncology, Gartnavel. Samples need to be analysed by the lab within 72 hours of sample collection-This is especially important for patients with a clinical history including Lymphoma	Samples to be placed in a purple specimen bag. Samples should be sent directly to Flow Cytometry laboratory by porter/courier: Haemato-Oncology, Gartnavel General Hospital, Paul O’Gorman Building, 21 Shelley Road, Glasgow, G12 0XB (0141 3017729)

Specimen types routinely processed by the Diagnostic Cytology Laboratory include:

Serous Fluids Cytology

Ascitic fluid, pericardial fluid, peritoneal washings and pleural fluid.

Respiratory Cytology

Bronchial aspirates, bronchial lavages, bronchial brushings and sputa.

Brushings Specimens

Pancreatic biliary, ureteric and common bile duct.

Fine Needle Aspiration (FNA) Cytology

Superficial FNA’s taken from thyroid, breast, lymph node, supraclavicular and submandibular. Endoscopic FNA’s including TBNAs (Transbronchial needle aspirations) from lymph nodes or masses, FNA from pancreas, stomach or oesophagus and fine needle biopsy (FNB) from pancreas/nodes.

Cerebrospinal Fluid (CSF)

CSF and other neuropathology fluids are processed by cytology and reported by the Neuropathology Consultants.

Synovial Fluids (NB: This test is not UKAS accredited)

Synovial fluids are examined for the presence of monosodium urate and calcium pyrophosphate crystals in cytology. Synovial fluids are also examined for microscopic assessment if septic arthritis, inflammation or infection is queried. Any Synovial fluid specimens where septic arthritis is suspected must have a separate additional specimen and request form being sent to Microbiology. Bursa fluid and ganglion fluid may be examined for the presence of crystals and microscopic cell evaluation.

Other Fluids

Cyst Fluids (e.g. ovarian cyst, parotid and Thyroid)

Urine

Urine, ureteric washings and renal pelvic washings. Cytology can test for the presence of casts in urine.

Certain specimens for Diagnostic Cytology can be transported by the previously listed normal specimen transport methods as well as the internal pneumatic tube system

Specimens from the Queen Elizabeth University Hospital may be sent via the internal pneumatic tube system. Only the following specimens may be sent via the internal pneumatic tube system: Fluids, FNAs which are fluids (Not slides), respiratory specimens and CSFs. Please do not send specimens with a danger of infection via the pneumatic tube system. Please ensure all specimens are sent in leak-proof containers (white lidded, screw top universal tubes are ideal).

The Diagnostic Cytology laboratory should be contacted on 89569 before a specimen is sent through the pneumatic tube system.  The pneumatic tube extension for cytology is 1616

The Diagnostic Cytology laboratory follows the same specimen acceptance criteria as the pathology department.

Specimens that are Mislabelled/Erroneous/Have Insufficient Information

In the event that a specimen received in diagnostic cytology where the specimen and/or the request form has been mislabelled or erroneous, the team leader or appropriate biomedical scientist will make every attempt to contact the sender and clarify what has happened.

This may be by email or by telephoning the clinician given on the request form.

If the event that there is insufficient information to contact the source regarding a specimen e.g. specimen sent with no request form. The specimen will be kept for 1 week, in the hope that the laboratory will be contacted. If no contact has been made at the end of this period, the specimen will be discarded.

A cytology trakcare request form should be sent for cytology tests not a histopathology trakcare request form. The cytology department does issue reports via trakcare.

Specimens taken specifically for the diagnosis of infectious diseases should NOT be sent to Cytology, but instead be sent directly to microbiology/virology as appropriate.

Specimens which are highly suspicious of, or from known Group 3 infectious diseases must be labelled as D.O.I

Examples of Group 3 Infectious Diseases

Coronavirus (COVID-19), Human Immunodeficiency Virus (HIV), Hepatitis C&D, Mycobacterium tuberculosis, and Creutzfeldt Jakob Disease (CJD)

CSF Cytology Specimens which are highly suspicious of CJD or known CJD should be labelled as D.O.I. A specimen should be sent directly to the Edinburgh CJD unit if there is a clinical suspicion of CJD.

Group 4 Infectious Diseases

Should not be sent to Cytology for processing. E.g. Viral haemorrhagic fever.

Specimens which pose a risk from infectious diseases must:

• Be placed in a D.O.I specimen bag (ONLY send D.O.I specimens in these bags)
• Have a D.O.I label on the Specimen Container
• Indicate there is a Danger of Infection by ticking the box on the request form
• Describe the nature of the risk (known or highly suspicious of) on the request form

Urgent specimens must be clearly labelled as urgent on the specimen request form.

The request form must also have the contact information of the clinician who requires the urgent result e.g.  telephone number or page of the clinician. If a specimen is required urgently for a Multi-disciplinary Meetings (MDT), please put this on the specimen request form and the date of the MDT.

https://www.nhsggc.scot/download/fna-labelling-guide

Name of Test	Specimen/Container Requirements:	Further Information:
Cervical Cytology/HPV Screening LBC Smears	The specimen should be taken following the normal smear taking protocol. The collected specimen should then be rinsed out into a Hologic™ Thin Prep Pap Test (LBC) specimen vial Please ensure the vial is within the date specified. If it is out of date then it must NOT be used.	Each LBC sample should be placed in a clear polythene bag and sent to the laboratory in the appropriate white cervical cytology samples only (LBC) bag and accompanied with a Specimen Dispatch Note. For general/technical enquiries please contact: Gillian Collins: 0141 354 (8)9501 Lab: 0141 354 (8)9524

The pathology department offers a service for immunofluorescence testing on skin, renal, conjunctival and oral biopsies.

Skin, Renal, Oral and Conjunctival Biopsies requiring immunofluorescence should be placed in Michel’s fixative immediately after collection.

Michel’s media is capable of preserving fresh tissue for up to 5 days before there is any deterioration in the sample. The request form is checked for the date a sample is taken, to ensure that it has been received within time. Michel’s media is available from the pathology department upon request. The fixative should be stored between 2-25⁰c and not be used after it has reached its expiry date.

COVID-19

Fresh samples are processed routinely for immunofluorescence analysis. However, if the patient is known to be COVID Positive, please can this be indicated clearly on the form so the laboratory will be able to take the appropriate precaution when processing these samples.

Danger of Infection

Specimens that are suspected or are known to contain blood borne viruses (e.g. HIV, Hep B, and Hep C) are classified as Category 2 and can be dealt with using the appropriate safety precautions.

Specimens suspected or known to have Category 3 organisms (e.g. TB, Leprosy, and Anthrax) or Category 4 organisms (e.g. Ebola, Lassa Fever and Haemorrhagic Fever) or CJD are not suitable for analysis via immunofluorescence.

For further information regarding immunofluorescence or danger of infection samples, please contact the Immunocytochemistry Department of the pathology laboratory (0141 354 9518 or 89518).

CHI/Unique identifier, Surname & Forename or if no CHI/unique identifier, the Surname, Forename and Date of Birth must be provided.

A minimum of 3 matching patient identifiers across both the specimen pot labels and forms are required to accept a specimen and meet legislative requirements.

Only Specimen containers and Forms that satisfy the essential criteria listed below can be accepted into the department.

Request Form

Essential:-

• CHI Number or a temporary unique identifier (e.g. ZP number)
• Patient’s Full Name or unique coded identifier
• Date of Birth
• Nature of Specimen including qualifying details (Any Danger of Infection information is essential)

Desirable:-

• Patient’s Address including Postcode (essential if no CHI number)
• Clinical Information/history including risk/danger of infection (essential for fresh tissue and frozen section)
• Date and time of sampling
• Practitioner’s contact number (bleep or extension)
• Gender
• Location and destination of report
• Requesting Practitioner
• Investigation Required

Sample Container

Essential:-

• CHI number or a temporary unique identifier (e.g. ZP number)
• Patient’s Full name or unique coded identifier
• Date of Birth
• Nature of Specimen or identifier including qualifying details
• Labelling to indicate if there is a risk/danger of infection

Desirable:-

• Date and Time of Sampling

Other Essential Information

The full address to which the report should be sent must also be included.

N.B: Pre-printed labels do not indicate the address to which the report should be sent.

A short clinical history must also be provided.

A brief clinical history is invaluable in the interpretation of the histological findings and should be given in every case.

As many pathology cases require considerable clinico-pathological discussion before a diagnosis can be reached, it is essential that the name of the patient’s consultant, the name of the requesting doctor and the contact telephone or page numbers are put on the request form where asked.

If the report is required urgently this should be stated on the request form along with a contact telephone number or page number.

The laboratory cannot supply a report on unlabelled specimens or specimens received without request forms.

Failure to provide the required information may result in delays in processing specimens and/or in the provision of a Pathology report to the appropriate location.

Normally, specimens should be transferred in a sealed container with 10% neutral buffered formalin, which prevents tissue decay, ideally the volume of formalin should be at least 10 times the volume of the specimen, but for major resections this may not be possible. Please ensure that containers are properly sealed before dispatch as leaking specimens will not be picked up by Transport/ Facilities staff. If you have a Formalin spill, follow your own spill procedures. 

No fixative should be added if:

• a frozen section is requested (including: brain biopsies taken mid surgery for smear analysis, muscle biopsies and paediatric rectal biopsies – see individual sections for more information)
• the specimen is thought to be a lymphoma – whole lymph nodes only
• there is a need to collect and process fresh surplus tissue for medical research purposes
• The sample is for Immunofluorescence 

Sending Fresh Tissue

All fresh tissue must be delivered to the Pathology Department immediately and Pathology Specimen Reception should be notified that fresh tissue has been sent.

Tel:  0141 354 9513 (89513) or 0141 354 9514 (89514)

If you are unsure of the correct procedure, contact Pathology Specimen Reception for advice on the telephone numbers above.

Delay in receipt of a “fresh” specimen may make diagnosis impossible.

Fixatives and specimen jars are available from:

Specimen Reception (L3/B/021),

NHSGGC Pathology Department

Level 3, Laboratory Medicine and Facilities Management Building

Queen Elizabeth University Hospital

Tel:  0141 354 9513 (89513) or 0141 354 9514 (89514)

Requests for urgent results should be clearly marked on the request form and contact telephone or page number provided.

The consultant pathologist should be notified before the specimen is sent if an urgent result is required.

It is the responsibility of the clinical team to ensure that emergency/urgent specimens are transported urgently to the Pathology Laboratory.

In practice, these are specimens that carry the risk of transmitting Hepatitis B virus, Hepatitis C virus, HIV, M. tuberculosis and other category III pathogens.

The Clinical Microbiology Department should be contacted where there is any uncertainty.

Such specimens, include those from confirmed or suspected cases of the disease, known carriers (e.g. those known to be hepatitis antibody or antigen positive), as well as patients from an ‘at risk’ group (e.g. drug abusers).

‘Danger of infection’ (DOI) stickers must be put on the specimen bag, the request form and the specimen container.

If the nature of the DOI is known please include this in the clinical details.

Specimen bags should be sealed so that they can be opened without the use of sharp or pointed instruments.

For large specimens, containers should be enclosed in individual plastic sacks tied at the neck. The request form should be placed in a plastic envelope which is then securely tied to the neck of the sack.

“Danger of Infection” specimens should always be sent in an adequate volume of buffered formalin.

If a specimen is received in Pathology and the specimen and/or request form has been mislabelled, is unlabelled or there are discrepancies between the request form and specimen container, the Laboratory staff will make every attempt to contact the sender to clarify the error, including telephoning the source and contacting requesting clinician etc. identified from any information that has been supplied.

If the Laboratory staff are unable to rectify the problem by telephone, a “Non-conformance letter” will be sent to the requesting clinician.

This letter is accompanied by the request form and identifies the nature of the non-conformance with a request that this is rectified, and the requesting clinician takes responsibility for the changes.

Specimens will not be returned but will be kept in Pathology Specimen Reception labelled with the appropriate non-conformance number, until the non-conformance has been resolved.

The laboratory cannot supply a report on unlabelled specimens or specimens received without request forms. Failure to provide the required information may result in delays in processing specimens and/or in the provision of a pathology report to the appropriate location.

In the event of an urgent specimen being received in diagnostic cytology, where the specimen and/or request form has been mislabelled, the technical staff in the lab will make every attempt to contact the sender and clarify the mislabelling error, including telephoning the source and contacting the requesting clinician etc. identified from any information that has been supplied.

In the event of there being insufficient information to allow cytology to contact the sender, the specimen will be kept refrigerated for one week, in the hope that the lab will be contacted. If no contact has been made at the end of this period, the specimen will be discarded.

NHSGGC Pathology Department Terms of Service

NHSGGC Pathology User Manual

NHSGGC Pathology Request Form

NHSGGC Placenta Request Form

NHSGGC Muscle Biopsy Request Form

NHSGGC Pathology Specimen Dispatch Note

NHSGGC Transport & Disposal of Specimen Containers & Specimens Policy

NHSGGC Confidentiality and Data Protection Policy

	External	Internal
Dr Sylvia Wright – Head of Service	0141 354 9512	89512
Dr Jana Crosby – Clinical Lead	0141 354 9558	89558
Dr Jonathan Salmond – Technology Lead	0141 354 9561	89561
Steven Harrower – Head of Technical Services	0141 354 9468	89468
Suzanne Ferra – Cellular Pathology Operations Manager	0141 354  9469	89469
Nicola Small – Compliance and Transformation Manager	0141 354 9461	89461
Vacant – Quality Manager	0141 354 9540	89540
Robert Cast – Mortuary Services Manager	0141 451 5815	85815
Deborah Brown – Mortuary Post Mortem Manager	0141 451 5795	85795
Vacant – Mortuary Scheduling & Performance Manager
Bio-repository Office	0141 354 9490	89490
Sarah Gilmour – Office Manager	0141 354 9568	89568
Histology Specimen Reception	0141 354 9513 or 0141 354 9514	89513 or 89514
Cytology Specimen Reception	0141 354 9524	89524
EM Enquiries	0141 354 9422	89422
SCRRS Enquiries	0141 354 9524	89524

The pathologist named first in each team, is the designated specialty representative.

Team	Members	Team	Members
Autopsy	Dr S. Fraser Dr K.Kinch Dr O.McCabe Dr J. Paxton Dr K. Tilley Dr S.Wright	Bone & Soft Tissue	Dr E. Macduff Dr S. Dundas Dr F. Roberts Dr K.Teo Dr A. Young
Breast	Dr E. Mallon Dr C. Dick Dr D. Kipgen Dr J. Loane Dr E. Macduff Dr A. Milne Dr S. Syed	Cardiovascular	Dr S. Wright Dr D. Kipgen
Dermatopathology	Dr L. Melly Dr S. Digby Dr C. Harper Dr G. Kohnen Dr V. Lynch Dr A. Milne Dr C Moyes Dr D. O’Dwyer Dr M. Paul Dr K. Tilley Dr A. Young	Diagnostic (Non-Gyn) Cytology	Dr C. Van der Horst Dr F. Duthie Dr C. Harper Dr D. Kipgen Dr A. Latimer Dr J. Slavin Dr S.Wright
Gastrointestinal (GI)	Dr F. Duthie Dr S. Bell Dr C. Dick Dr S. Fraser Dr C. Harper Dr G. Kohnen Dr P. Konanahalli Dr S. Liptrot Dr E. MacDuff Dr N. Maka Dr O. McCabe Dr K. Myint Dr K. Oien Dr H. Pitchamuthu Dr F. Roberts Dr J. Salmond Dr J. Slavin Dr G.Smith Dr K. Tilley	Gynaecology	Dr S. Bell Dr G. Bryson Dr D O’Dwyer Dr G. Kohnen Dr P. Konanahalli Dr S. Syed
Cervical Cytology	Dr A. Latimer Dr S. Liptrot Dr S. Syed Dr C. Van der Horst	Haemato-Lymphoid	Dr J. Goodlad Dr C. Harper Dr S. Liptrot Dr K. Myint Dr J. Paxton
Head & Neck/Endocrine	Dr L. Cooper Dr S. Dundas Dr O. McCabe Dr K. Myint Dr J. Slavin Dr S. Wright	Liver	Dr G. Kohnen Dr K. Oien Dr P. Konanahalli
Neuropathology	Dr A. Stan Dr Z. Hanzely Dr W. Stewart	Ophthalmic	Dr F. Roberts Dr C. Thum
Paediatric	Dr C. Evans Dr S. Bitetti Dr P. French Dr D. Penman	Renal	Dr D. Kipgen Dr J. Crosby
Respiratory	Dr C. Dick Dr E. MacDuff Dr H. Pitchamuthu Dr F. Roberts Dr J. Slavin	Urology	Dr V. Lynch Dr G. Bryson Dr J. Crosby Dr S. Fraser Dr H. Pitchamuthu Dr J. Salmond

We invite all our users to complete our user survey form. Please return via email to the Compliance and Transformation Manager. The information obtained from this survey will allow us to develop and improve the service we offer. We greatly appreciate the time and effort taken to complete this.

For general complaints/compliments/comments on the service please contact:

Dr Sylvia Wright (Head of Service) 

Tel: 0141 354 9558

Mrs Nicola Small (Compliance and Transformation Manager)

Tel: 0141 354 9461

Please click here for further information about the NHSGGC Complaints policy

HCSWs work in many different clinical areas delivering safe, effective, person-centred care. These areas include inpatient wards, outpatient areas, emergency departments, critical care and theatres.

Although these resources refer to inpatient care, they have relevance to all HCSWs new to our organisation.

Induction Essential Learning

HCSW Fundamental Care Presentation

Preparing for work in health and social care | Turas | Learn (nhs.scot) 

Complete the modules: 

• Who’s who in health and social care
• Bed making

Person-centred care is about putting the person at the heart of their care. By asking and listening, we can understand more about the person and do the things that are most important to them. This helps us to provide care that is individualised and improves their experience of care. Delivering high-quality, person-centred care is everyone’s business in NHSGGC. 

Induction Essential Learning 

Delivering high quality person-centred care

Enable external content to view the short videos.  

Other Useful Resources 

Communicating with and supporting people: information for support workers in health and care settings | Turas | Learn (nhs.scot) 

Person Centred Care Made Simple – The Health Foundation

Food and fluid gives our body energy to allow us to carry out our everyday tasks. Delivering the right amount of food and fluid to our patients is an important role of the HCSW. In your first few shifts on the ward, work with the mealtime coordinator at meal service and find out what’s available for patients, and how to help them with their meals. By supporting people to eat and drink we can help them recover from their illness or surgery and get out of hospital sooner.

Induction Essential Learning

Food, Fluid and Nutrition (Presentation) 

Other Useful Resources

Food, Fluid and Nutrition Manual (Staffnet- Access from GGC Computer)

A pressure ulcer is an area of skin damaged by pressure. It can be caused by sitting or lying in one position for too long or by rubbing or dragging skin across a surface.  The risk of developing a pressure ulcer increases when a person has problems with walking, using the bathroom or they don’t eat and drink enough. It is important that the skin is kept clean and dry and if there are any devices (such as a splint or catheter) the skin under and around these are checked regularly to make sure no damage is developing.

Pressure ulcers can develop very quickly. There are simple measures that you can do to help reduce the risk of your patients developing a pressure ulcer including regular observation of their skin and making sure that they are not sitting or lying in the one position for too long and writing this on the Care Rounding Chart. Your colleagues may refer to pressure ulcer prevention as ‘Tissue Viability’.

Induction Essential Learning

learnPro® module GGC: 080 Prevention of Pressure Ulcers

Other Useful Resources

Tissue Viability Resource Folder (Acute) Staffnet- Accessed on GGC Computer only

Infection control is everyone’s business and we all have a part to play in keeping both ourselves and the people we look after safe. People with infection are more likely to come to harm, stay in hospital longer and may even die as a result.  Keeping your hands clean, washing them regularly and using personal protective equipment (known as PPE) are important in stopping the spread of infection. In healthcare, Hand Hygiene and PPE are two of the 10 Standard Infection Control Precautions (known as SICPs), which are described in more detail in the GGC:007 Statutory Mandatory Modules.

Induction Essential Learning

learnPro® module GGC:007 Standard Infection Control Precautions 

learnPro® module NES: Prevention & Control Infection: C.Difficile 

Put on (‘donning’) and take off (‘doffing’) PPE Please note that as we move to business as usual following the pandemic, donning and doffing remains relevant for many organisms.

Standard Infection Control Precautions (SICPs) may be insufficient to prevent cross transmission of specific infectious agents. Therefore, additional precautions, Transmission Based Precautions (TBPs), are required to be used by staff when caring for patients with a known or suspected infection or colonisation. More detail is available in the National Infection Prevention and Control Manual: Chapter 2 – Transmission Based Precautions (TBPs) (scot.nhs.uk). Speak to your line manager about when TBPs may be required and the PPE requirements for these specific patients that require them. 

Other Useful Resources

Infection Prevention and Control – NHSGGC Homepage

National Infection Prevention and Control Manual

Education Strategy – NHSGGC

learnPro® NES: Scottish IPC Education Pathway – Foundation (Infection Prevention & Control tab)

This outlines the minimum requirements of a Paediatric HCSW to support safe, effective, person centred palliative care within NHSGGC. The Paediatric Supportive and Palliative Care team at Royal Hospital for Children (RHC) provide tertiary specialist paediatric palliative care across the paediatric spectrum, from the antenatal period, through infancy and childhood and also for young people until they reach their 16th birthday (and those who are 17-18 years but still accessing services at RHC). The service provides an individual patient-centred pathway from diagnosis or recognition that a condition is life-threatening or life-limiting.

The team will accept referrals from any healthcare professional. If a patient requires to be assessed by a member of the team the named consultant for the child or young person’s care must give approval. Get in touch with the team with an electronic referral via TrakCare® – Paediatric Supportive and Palliative Care consult.

Induction Essential Learning

Babies,Children and Young People’s Palliative Care 

Other Useful Resources

Palliative Care Resource Folder (Neonatal and Children’s services)(Access from a GGC computer)

or click on the pink leaf tile on the right hand side of Staffnet homepage

Moving and handling activities is a key part of your duties. This includes assisting patients and tasks such as moving equipment, laundry and stores. To keep yourself and patients safe it is essential you have the correct level of training.

If you have a Scottish Manual Handling Passport  no foundation practical training course is required. Bring the passport document in to show your SCN or Team Lead. For Registered Nurses on the NHSGGC Staff Bank, send a copy by email to adminstaffbank@ggc.scot.nhs.uk. The dates of Moving and Handling training documented in the passport will go into the ward/ department training records. Complete the mandatory learnPro module: 005 Manual Handling Theory together with the practical competency assessment within your clinical area carried out by one of the local assessors.

If you do not have a Scottish Manual Handling Passport complete learnPro module: 005 Manual Handling Theory and your SCN or Team Lead will nominate you for the foundation practical training course (1day).  Whilst waiting for your training course please review the NHSGGC Moving and Handling information page.

All patients should be verbally encouraged to move and position themselves independently. When a patient needs to be moved, handled or requiring a mechanical aid this should be carried out with a member of the ward / department team and not on your own. Please, do not to use hoisting equipment until training completed.

You may come across emergency situations when you are at work. Patients can have problems with their airway, breathing or circulation and become suddenly unwell. Your SCN will nominate you for a short course called Basic Life Support which will help you to respond correctly in an emergency situation.

As part of your orientation to your ward / clinical area take some time to locate the emergency / resuscitation trolley 

If you find yourself in an emergency situation you may be asked to phone for the resuscitation team. Dial 2222- ask for the Paediatric Resuscitation Team and tell them your clinical area and the hospital you are in. 

To prevent needlestick injuries, NHSGGC provides sharp safe devices across the organisation within all areas of clinical practice, for the protection of staff.

Induction Essential Learning 

If you use sharps as part of your role you must complete:

LearnPro® module: Prevention and Management of Occupational Exposure (Scottish IPC  Education Pathway – Infection Prevention and Control tab)  

Other Useful Resources

Sharps – NHSGGC